H9164
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 | Immunoblotting Cell lysates were separated on SDS-PAGE and blotted with Anti-Acetyl-Histone H4 [Ac-Lys16] (Cat. No. H9164). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:2,000. Lanes 1,3,4. HL60 2. HeLa 3. No inhibition with Histone H4 (human, 9-18) (2 μg/mL) 4. Inhibition with Acetyl-Histone H4 [Ac-Lys16] (human, 9-18) immunizing peptide (2 μg/mL) |
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H9164
Sigma
Anti-acetyl-Histone H4 (Ac-Lys16) antibody produced in rabbit
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Description
| Immunogen | synthetic peptide corresponding to amino acids 9-18 (Ac-Lys16) of human histone H4, conjugated to KLH. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~12 kDa |
| species reactivity | human |
| concentration | ~1.5 mg/mL |
| application(s) | immunoblotting: 1-2 μg/mL using HL60 cell lysates |
| Gene Information | human ... HIST4H4(121504) |
| shipped in | dry ice |
| storage temp. | −20°C |
